D. B. Western Inc

Robert W, R. Humphreys received his B,Sc, andPh.D. in Photochemistry and ESR Spectroscopy with Professor Donald R. Arnold at the University of Western Ontario. After 1 1/2 years postdoctoral work with Professor Cheves Walling at the University of Utah, he worked as a Research Chemist at Loctite Corporation in anaerobic adhesives chemistry. He recently joined Lever Research, Inc. as a Research Scientist. His current research interests include free radical and peroxide chemistry and metal catalyzed redox reactions. 

Figure 1. Purification of pea carbonic anhydrase. Tb figure shows a Coomassie Blue stained, 7-15% lineaf gradient, SDS polyacrlyamide gel (PAG) loaded with total soluble protein (lane G), 30-60% (NH4)2S04 precipitate (lane F), eluate from the affinity columt chromatography (lane E) and after excision and electroelution from an SDS-PAG of the affinity column eluate (lane D). Lane C contains the polypeptides found in fractions exhibiting CA activity after Sephadex G-200 chromatography of the affinity column eluate and lane B contains polypeptides electroeluted from a section of a native PAG stained for CA activity. A Western blot of the total soluble protein (lane G) probed with the CA polyclonal antisera is shown in lane I. Molecular weight markers (Bio-Rad Inc.) are shown in lanes A and H...

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