Czapek Dox agar

Two typical media for growth of fungi are Sabouraud liquid medium and Czapek-Dox medium. Both these media may be sohdified with agar if required. 

A spore sand culture containing Gibberella zeae (Gordon) NRRL-2830 was aseptically placed in a sterile tube containing 15 ml of Czapek s-Dox solution and a small amount of agar. This medium was then incubated for about 168 hours at approximately 25°C. At the end of the incubation period, the medium was washed with 5 ml of steriledeionized water and transferred to a sterile tube containing 45 ml of Czapek s-Dox solution. The contents of the tube were then incubated for about 96 hours at about 25°C after which the material was available for use in inoculation of a fermentation medium. 

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