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Cytosolic simultaneous measurement with

FIG. 5. Simultaneous and reproducible measurements of changes in SR and cytosolic Ca2+ in response to agonists. Pregnant rat myometrial cells were superfused with Krebs (2 mM Ca2+) and Carbachol (in Ca-free solution) applied as indicated. Repetitive applications of Carbachol evoked reproducible [Ca2+]j elevations (bottom trace) and SR [Ca2+]L decreases (upper traces). Left-hand axis cytosolic Ca2+. Right-hand axis intraluminal (SR) Ca2+changes, measured with mag-fluo-4. (Taken from Shmigol et al 2001.)... [Pg.15]

The isolated renal vessel technique has been modified so that vessels can be isolated from rats instead of rabbits [256]. The preparation has also been modified for fluorescence measurements of cytosolic calcium in the smooth muscle cell layer [257]. Measurements of changes in smooth muscle calcium can be carried out simultaneously with determinations of changes in lumen diameters. The effects of various agonists and pressure stimulation in the presence and absence of pharmacologic agents can be determined. Angiotensin... [Pg.101]

See other pages where Cytosolic simultaneous measurement with is mentioned: [Pg.195]    [Pg.163]    [Pg.83]    [Pg.14]    [Pg.52]    [Pg.258]    [Pg.191]    [Pg.150]    [Pg.355]    [Pg.327]   
See also in sourсe #XX -- [ Pg.2 , Pg.14 , Pg.15 ]



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Cytosol

Cytosolic

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Simultaneous measurements

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