Cytokinins 2,4-dichlorophenoxyacetic acid

The leaf segments ca. 5x5 mm) of axenic shoots were cultured on MS solid medium supplemented with auxin or combinations of auxin and cytokinin at 25 C in the dark. Calli were formed at the cut end of leaf segments within 2 to 3 weeks on MS solid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D, 1-3 mg/1) and Kin (0-0.1 mg/1), while adventitious roots appeared after intermediary callus on MS solid medium containing either lAA (1-3 mg/1), NAA (0.5-3 mg/1), or NAA (1 mg/1) plus Kin (0.1 mg/1). The calli and adventitious roots grew well and could be maintained in the same cultural conditions as was used for their induction. Fig. (28). 

Balasimha D, Ram G, Tewari MN (1977) Cytokinin-coumarin interaction in relation to growth, sulfhydryl, chlorophylls, and peroxidase-activity in Phaseolus radiatus L. seedlings. Biochem Physiol Pllanz 171 49-54 Ball NG, Dyke IJ (1956) The effects of indoie-3-acetic acid and 2 4-dichlorophenoxyacetic acid on the growth rate and endogenous rhythm of intact Avena coleoptiles. J Exp Bot7 25-41... 

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