Cytidine deaminase immobilization

For further scale-up, the use of immobilized enzyme was highly desirable. The potential for reuse of immobilized cytidine deaminase was shown by a series of small-scale... 

Using this approach, 20 kg of optically pure Epivir was isolated to support the development program. For further scale-up. the process was transferred to our production site at Ulverston, Cumbria, where several tons of Epivir were produced using immobilized cytidine deaminase from the recombinant strain. 

Figure 11 Emymatic resolucion of ) 10 at 3 kg scale using immobilized cytidine deaminase. Three kilograms of racemic 10 was incubated at 32 with 30,000 units of immobilized cytidine deaminase in a 300 liter volume in a 780 liter fermentor. Tlie pH was adjusted to 7.0 and maiiv mined constant by the addition of 20% (v/v) acetic acid. Upon completion of the reaction, the mlxtute was pumped out through a 0.1 mm steel mesh, and the retained beads were stored at 4°C. ( ) pH ( ) conversion % deamination of racemic 10). (Reproduced from Ref. 57 with the permission of Buttetwonh Heinemann.)...

Due to previous work with immobilized adenosine deaminase, we chose to use Eupergit-C (RKom Pharma) as a stable, robust support for immobilization of cytidine... 

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