Cyclopenin m-hydroxylase

Fig. 10. In vitro activities of the enzymes of alkaloid biosynthesis and rates of alkaloid formation in hyphae of Penicitlium cyclopium (75). Cultivation conditions were as described in the legend to Fig. 9. At the times indicated by arrows, cycloheximide (100 p.g/ml) was added to the culture medium. All values are in units per 1 cm of culture area. (A) Anthranilate adenylyltransferase (AA) (100 = 5.6 pkat) ( ) cyclopeptine dehydrogenase (CD) (100 = 40 pkat) ( ) dehydrocyclopeptine epoxidase (DE) (100 = 0.42 pkat) (A) cyclopenin m-hydroxylase (CH) (100 = 12 pkat) (O) cyclopenin-cyclopenol formation in vivo (100 = 9 pmol/sec).

Figure 3.61 Biosynthetic pathway to cyclopenin and cyclopenol, and their conversion to viridicatin and viridicatol, respectively, in Penicillium cyclopium. (i) cyclopeptinsynthase complex (ii) cyclopeptin dehydrogenase (iii) dehydrocyclopeptin epoxidase (iv) cyclopenin m-hydroxylase (v) cyclopenase.

Cyclopeptine synthetase 2 cyclopeptine dehydrogenase 3 dehydrocyclopeptine epoxidase 4 cyclopenin m-hydroxylase 5 cyclopenase... 

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