Amine-reactive Cyanine Dyes

DNA modified with a diamine compound to contain terminal primary amines may be coupled with amine-reactive fluorescent labels. The most common fluorophores used for oligonucleotide labeling are the cyanine dyes and derivatives of fluorescein and rhodamine (Chapter 9). However, any of the amine-reactive labels discussed throughout Chapter 9 are valid candidates for DNA applications. 

D-differential imaging gel electrophoresis (DIGE) is also a gel-based procedure, but one that is more precise than the 2-DE approach. A key element of this procedure is labeling of proteins in the two experimental samples with different colored amine reactive fluorescent dyes.46 47 A common procedure is to label the control sample with Cy-5 cyanine dye and the test sample with Cy-3 cyanine dye. These two dyes display fluorescence at 670 and 570 nm, respectively. The two samples are then mixed in equal concentrations and separated... 

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