Current chromatogram, lipid

Figure 5 Total ion current chromatogram of acylglycerols 2h after administration of structured triacylglycerols (A) 1,3-dioctanoyl-2-linoleoyl-sn-glycerol (8 0/18 2/8 0), (B) 1,3-did-ecanoyl-2-linoleoyl-sn-glycerol (10 0/18 2/10 0), and (C) 1,3-didodecanoyl-2-linoleoyl-sr>-glycerol (12 0/18 2/12 0), and of safflower oil (D). All the triacylglycerols were identified by atmospheric pressure chemical ionization (APCI)-LC-MS. Reproduced with permissbn from Mu H and Hoy C-E (2001) Journal of Lipid Research 42 792-798.

Among these three conditions, only the first approach or its variations was used widely in practice. Accordingly, a total ion current chromatogram of each individual molecular ion can be extracted (i.e., selected-ion monitoring) from the recorded mass spectra in the full MS mode from an HPLC run. Indeed, many ions of interest can be extracted simultaneously in this way [51]. To perform quantitative analysis of lipids, the linear dynamic range, limit of detection, and calibration curves of the molecular species of interest are generally predetermined. Thus, the reconstituted ion peak area of each species can be compared to a standard curve obtained under identical experimental conditions. 

Figure 11.4 Total ion current (TIC) chromatogram of lipid residue extracted from a potsherd of Early Neolithic date (Ecsegfalva, Hungary). Cx y F refer to fatty acids with carbon number (x) and number of unsaturations (y). Cx T refer to triacylglycerols with number of carbon atoms (x). P = plasticizer contamination. (Craig et al., 2007, 354 Figure 18.1, by permission.)...

Figure 6.2 (a) Total ion current (TIC) chromatogram of Dictyostelium discoideum total lipid... 

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