Cryomicroscopy analysis

Suzuki, H., Yonekura, K., and Namba, K. (2004). Structure of the rotor of the bacterial flagellar motor revealed by electron cryomicroscopy and single-particle image analysis./. Mol. Biol. 337, 105-113. 

Baker, M. L. (2002). Development and Applications of Intermediate Resolution Structural Analysis Tools Integrating Bioinformatics and Electron Cryomicroscopy. Ph.D. thesis. Baylor College of Medicine, Houston, TX. 

A comparison of literature values of subzero collapse transition temperatures (Tc) for an extensive list of water-compatible monomers and polymers has established the fundamental identity of Tg with the minimum onset temperatures observed for various structural collapse and recrystallization processes (1.2.115), for both model solutions and real systems of foods, as well as pharmaceuticals and biologicals (38.42.106.108.109). The Tc and Tr values of frozen or vitrified samples, tabulated elsewhere (1.2.22). are typically determined by cryomicroscopy, thermal analysis, or electrical resistance measurements (22 and refs, therein), on an experimental time scale similar to that for Tg by our DSC method (106.109). A comprehensive list of collapse processes (Table I), all of which are governed by Tg of frozen systems (or a higher Tg pertaining to low moisture systems processed or stored at T > 0 C) and involve potentially detrimental plasticization by water, has been identified and elucidated (1-5.18-... 

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