Croton cuneatus

A dereplication example that illustrates the use of microscale derivatization and analysis by GC-MS is an identification of a series of bioactive diterpenes by NCI researchers (35). The extract from Croton cuneatus showed activity in a phorbol dibutyrate receptor, binding assay, and, on analysis, could be separated by HPLC into a group of seven very minor, related components. Following hydrolysis and acetylation, all seven components appeared to give rise to only two peaks, which by GC-CIMS analysis were judged to be phorbol tetraacetates. It was concluded that the seven compounds were probably van-ous esters of 4a-, or 16-hydroxylated phorbol analogs (Fig. 6). 

Dereplication of phorbol bioactives Lyngbya majuscula and Croton cuneatus J. Nat. Prod. 53, 867—874. 

Croton cuneatus, C. lechleri, C. palanostigma, C. trinitatis SV, MCMV Virucidal [11]... 

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