Crosslinking agents disulfide containing

Figure 8.1 Cleavage of disulfide-containing crosslinking compounds can be done using a reducing agent such as DTT. Reduction causes the conjugates to break apart into their original components with each component containing a portion of the crosslinker that terminates in a thiol group.

In silica reinforcement systems containing TESPT, Wolff has suggested that the reaction is similar when the TESPT/silica intermediate is present instead of sulfur, in which case the crosslinking agent is the polysulfidic sulfur chain. Wolff showed that mercaptobenzothiazyl disulfide (MBTS) reacts with the tetrasulfane group, thus forming 2 mol of the polysulfide ... 

Figure 4.17 DPDPB is a sulfhydryl-reactive crosslinker that forms disulfide bonds with thiol-containing molecules. The conjugates may be disrupted using a disulfide reducing agent such as DTT.

SADP, N-succinimidyl-(4-azidophenyl)l,3 -dithiopropionate, is a photoreactive heterobifunctional crosslinker that is cleavable by treatment with a disulfide reducing agent (Thermo Fisher). The crosslinker contains an amine-reactive NHS ester and a photoactivatable phenyl azide group, providing specific, directed coupling at one end and nonselective insertion capability at the other end. 

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