Crosslinked enzyme aggregate

Mateo, C., et al. (2004) A new, mild crosslinking methodology to prepare crosslinked enzyme aggregates. Biotechnol. Bioeng., 86, 273-276. 

Protein-polymeric membrane in a microchannel is prepared by using a concentric laminar flow (Fig. 43) [267]. Crosslinking condensation of a crosslinked enzyme aggregate (CLEA) [268] with aldehyde groups, which react with amino groups of the enzyme, in a concentric laminar flow results in the formation of a cylindrical enzyme-polymerized membrane on the inner wall of the microtube. The use of this technology for membrane formation in a microchannel can be extended to a broad range of functional proteins. 

Martinez YN, CaveUo I, Cavalitto S, lUanes A, Castro GR. Studies on PVA Pectin cryogek containing crosslinked enzyme aggregates of keratinase. Colloids SurfB Biointerfaces 2014 17 284-9. 

Vaidya, A. Xie, W. Gao, W. Bohling, J.C. Miller, M.E. Gross, R. A. Enzyme immobilization without a support Candida antartica lipase B (CALB) Self-crosslinked aggregates ACS Polymer Preprints, 2006, 47(2), 236. 

The monomer of the polymer is mixed with the enzyme solution to aggregate with the aid of polymerization accelerator (including the crosslinking agent). 

The elution time of the mutant carboxylase by analytical gel filtration is significantly reduced, relative to that of the wild type enzyme, a dimer of large sub mits (Fig.2). Analysis in denaturing conditions demonstrates that the mutant protein is a polymer of large subunits. Comparison with the elution profile of aggregates of crosslinked by disulphide bridges shows that the mutant elutes within the range of an octomer Lg (Fig.2). 

Wilson L, Betancor L, Fernandez-Lorente G, Puentes M, Hidalgo A, Guisan JM, et al. Crosslinked aggregates of multimeric enzymes a simple and efficient methodology to stabilize their quaternary structure. Biomacromolecules 2004 5 814-7. 

FIGURE 16.10 (a) Crosslinking AuNPs assembly approach and (b) non-crosslinking approach using AuNP aggregation for sensing enzyme activity. ... 

---