Cross-bridge cycling kinetics

Over the last twenty years biophysical work on this preparation has concentrated mainly on the elucidation of the filament structure and cross-bridge conformations (Reedy et al., 1965 Squire et al., 1977 Wray, 1979 Clarke et al., 1986 Reedy et al., 1987), and on the mechanical characterisation of various equilibrium states and of the kinetics of the cross-bridge cycle (Jewell Ruegg, 1%6 White, 1970 Tregear, 1977 Gtith et al., 1981 White Thorson, 1983). The biochemistry of ATP hydrolysis by the insect proteins has received less attention than that of vertebrate muscle proteins, primarily because of shortage of tissue, but recently aspects of the biochemical kinetics have been investigated (White et al., 1986). 

By using caged compounds in combination with permeabilized smooth muscle preparations, valuable information regarding the rate-limiting steps between excitation and contraction (see Somlyo and Somlyo, 1990, for review Somlyo et al., 1992), regulation of Ca + release from the SR (Walker et al., 1987 lino and Endo, 1992), and the kinetics of the cross-bridge cycle (Arner et al., 1987 Somlyo et al, 1988 Fuglsang et al,... 

Brenner B (1986) The cross-bridge cycle in muscle. Mechanical, biochemical, and structural studies on single skinned rabbit psoas fibers to characterize cross-bridge kinetics in muscle for correlation with the actomyosin-ATPase in solution. Basic Res Cardiol 81 (Suppll) 1-15... 

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