Covalent Binding to Liver Microsomes

Typically, the extent of covalent binding of microsomal protein may be evaluated by incubating or -labeled dmg substance with liver microsomes in the presence 

Data is expressed as picomolar equivalents per milligram of protein and is equivalent since the exact nature of the species bound at this point is unknown. Typically both human and rat liver microsomes would be used in order to get an estimate of bioactivation, since there may be substantial species differences. The rat data may be correlated with rat in vivo data to help contextualize the human data. 

Tkn assay using the hard nucleophile cyanide was described in Section 7.5.1.2, where non-radiolabeled KCN was used to detect the presence of cyanide adducts using mass spectrometry. However, by using K CN, it is possible to make this a quantitative assay by assessing the amount of cyanide incorporated into a non-radiolabeled test 

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