Coupled enzyme reactions in biosensors

Fig. 141. Coupling of molecular recognition and internal signal processing, e.g. amplification and chemical filtering, by coupled enzyme reactions in biosensors.

The third chapter concentrates on metabolism sensors, which are arranged according to the degree of biocatalyst integration. The various different ways of coupling enzymes with transducers in monoenzyme sensors are exemplified by the determination of glucose and urea. The current state of the art is shown for monoenzyme sensors for some further 25 analytes and classes of analytes. Coupled enzyme reactions are shown to provide expansion of the biosensor concept to new analytes and to multiparameter assays as well as to an improvement of such analytical parameters as specificity and sensitivity. This chapter offers for the first time a complete overview of the potentials of coupled enzyme reactions in biosensors. 

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