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Cosmid vectors packaging

Collins, J. Hohn, B. (1978). Cosmids a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage X heads. Proc. Natl. Acad. Sci. USA 75,4242-4246. [Pg.70]

Collins, J., and Hohn, B. (1978). Cosmids A Type of Plasmid Gene-Cloning Vector That Is Packageable in Vitro in Bacteriophage A Heads. Proc Natl Acad Sci 75 4242. [Pg.331]

Derivatives of phage A are able to act as vectors for much larger segments of DNA than is possible with plasmid vectors. However, the recombinant DNA must be packaged into the phage for amplification. Methods of in vitro packaging have been developed to yield up to 10 plaques per ftg of recombinant DNA. The in vitro packaging technique has permitted the construction and exploitation of totally novel vectors termed cosmids. These are plasmids into which the A cos site, at which... [Pg.142]

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See also in sourсe #XX -- [ Pg.151 ]



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