Copper-teicoplanin complex

Berthod et al. also tried copper complexation with teicoplanin and TAG CSPs [19]. Similar results were obtained. Amino acid enantiomers perfectly separated by both teicoplanin and TAG CSP could no longer be separated as soon as copper was present in the mobile phase. The copper-teicoplanin complex is also formed with the primary amine group on the peptidic teicoplanin basket (Figs. 1 and 3). However, unlike the vancomycin-copper complex which was very stable [18], the teicoplanin-copper complex was found to be reversible. Indeed, amino acid enan-tioselectivity was mostly restored after washing the chiral column with several column volumes of copper-free clean mobile phase [19]. 

Berthod A, VaUeix A, Uzon V, Leonce E, Caussignac C, Armstrong DW (2001) Retention and selectivity of teicoplanin stationary phases after copper complexation and isotopic exchange. Anal Chem 73 5499-5508... 

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