Contents 6 Aminophenols

Amine and Aminophenol Derivatives. Amines and aminophenols (Table VIII) react with the F-C reagent about as predicted considering the aromatic amino groups equivalent to phenolic hydroxyls. This would be an important interference with total phenol assay in samples with appreciable aromatic amine content. Fortunately, for this and other reasons as well, the major wine grapes and most other fruit and vegetable products are free of significant concentrations of aromatic amines which would interfere. Correction might be made for methyl anthranilate... 

Another method is extraction with methylene chloride followed by derivatization with pentafluorobenzyl bromide or diazomethane and snbseqnent GC/ECD or GC/FID analysis . The extraction solvent has to be changed before analysis. More than 50 substituted phenols have been derivatized successfully with Af-(f-butyldimethylsilyl)-Af-methyltrifluoroacetamide by forming the corresponding f-bntyldimethylsilyl derivatives. This study includes 21 chlorinated phenols, 13 nitrophenols, 3 aminophenols, 4 alkylphenols, o-phenylphenol, some other substitnted phenols inclnding 6 phenolic pesticides and the nonsnbstitnted phenol. Using SPE with polymeric adsorbents and GC/MS, phenols with very different substituents can be detected in environmental samples with high matrix content at the ppt level . 

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