Contact papain

Different enzymes have different specificities. Some, such as amylase, are specific for a single substrate, but others operate on a range of substrates. Papain, for instance, a globular protein of 212 amino acids isolated from papaya fruit, catalyzes the hydrolysis of many kinds of peptide bonds. In fact, it s this ability to hydrolyze peptide bonds that makes papain useful as a meat tenderizer and a cleaner for contact lenses. 

Papain Papain may be inactivated by the salts of heavy metals such as lead, silver, and mercury. Avoid contact with medications containing these metals. 

Allergan enzymatic contact lens cleaner Allergan Papain Plant Tablet... 

High performance liquid chromatography (HPLC) data showed that the reacety-lated chitosan was polydisperse, with fractions in the MW range 2 x 10 -1 X 10 Da (average 5 x 10 Da) after 10 min contact with papain, the average value was... 

In the control test, the enzyme having been immediately destroyed, the predpitate, weighing 338 mg. represents the quantity of total albumin contained in each tube. In test No. 2, the papain has been left i minute in direct contact with the albumin, then rapidly brought to 100 . The duration of heating, which has been from 10 to 20 seconds, has been quite sufiSd t to bring abouta maximum effect, ii 2 mg. of dissolved albumin... 

The digestive power of pepsin has then, been considerably diminished by contact with trypsin. Analogous results are found when pepsin and papain are left together either in neutral or in slightly acid media papain destroys or weakens the pepsin. 

Contact urticaria from papain has been reported by Baur et al. (1982) in papain workers, and from contact-lens cleansing solutions by Bernstein et al. (1984), Santucci et al. (1985) and Podmore and Storrs (1989). Papain persists in the soft lens matrix despite careful rinsing (Fichman et al. 1978). In a factory manufacturing papain, Baur et al. (1982) reported that 17 of 33 workers developed type-i reactions such as periorbital edema, conjunctival irritation or allergic asthma. 

B cells and B-LCL express surface immunoglobulin and this can be exploited to select cells secreting antibody with the desired specificity. For blood group antibodies, sterile red cells of the appropriate blood group are conveniently used. If the antigen is resistant to proteolytic enzymes (papain, bromelain, tiypsin) these can be used to reduce the surface charge of the cells which enhances contact with B cells. 

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