Construction and Expression of Fragmentary Enzyme

Although the overall conformation of the fragmentary enzyme appears to be indentical with that of the wild-type enzyme, it has only 40% of the activity of the native enzyme. The 

The relative activity of the fragmentary enzyme to the wild-type enzyme was much higher than that of the clipped to the native DadB enzyme. This was explained by the assumption that the thermostable enzyme has more extensive hydrophobic interdomain interactions than the DadB enzyme with less thermostability.12 The importance of hydrophobic interdomain interactions for catalysis was pointed out by studies on lactate dehydrogenase.23,24  

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