Conjugation multi-step protocols

The generalized protocol for performing a multi-step conjugation reaction with MBS or sulfo-MBS is similar to that described for SMCC (Section 1.3, this chapter). Specific examples may be found in the cited references. 

GMBS can be used in multi-step conjugation protocols wherein an amine-containing molecule or protein is first modified via the NHS ester end (its most labile-reactive group)to... 

Conjugation reactions done with SIAX or SIAXX are usually multi-step procedures similar to the protocol described for SLAB, previously. 

NPIA is water-insoluble and should be dissolved in DMF or methylene chloride prior to addition of an aliquot to an aqueous reaction medium. Conjugation reactions done with NPIA are usually multi-step procedures similar to the protocol described for SLAB, previously. 

The synthesis of oligomeric peralkylated polyaminophosphazenes based upon the P3 and P4 templates is an elegant (but complicated), hazardous, and expensive multi-step process. To exploit catalysts of this type in silicone synthesis on an industrial scale would require a much simplified and lower-cost synthetic protocol. One of the simplest and lowest-cost routes to a conjugated -P=N- template that forms the framework for the synthesis of phosphazene base materials is via phosphonitrilic chloride oligomers (Fig. 3). These are well-known acidic catalysts used in the silicone industry for the condensation polymerization of silanol-terminated polydimethylsiloxanes. Catalysts of this type are most commonly prepared by the reaction of PCI5 with NH4CI or HMDZ,... 

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