Conjugate aperture planes

Another way to suppress stray light is through field stops and aperture stops. A field stop is placed in a conjugate image plane of the excited spot in the sample. An aperture stop is placed most efficiently in the image plane of another aperture, which can be another stop, a lens, or a mirror. Two examples are shown in Fig. 7.29. A lens, LI, focuses a laser beam into a sample. The light emitted by the... 

Figure 2 Ray-paths in the transmitted-light microscope showing (A) the field set of planes that are conjugate with the object and the final image and (B) the aperture set of planes conjugate with the filament and the apertures of condenser and objective lenses. (Reproduced with permission from Bradbury S, Evennett PJ, Haselmann H, and Piller H (1989) RMS Dictionary of Light Microscopy. Oxford Oxford University Press and Royal Microscopical Society.)...

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