Computer simulations isoelectric focusing

Bier, M Mosher, RA Palusinski, OA, Computer Simulation and Experimental Validation of Isoelectric Focusing in Ampholine-Free Systems, Journal of Chromatography 211, 313, 1981. Bier, M Palusinski, OA Mosher, RA Saville, DA, Electrophoresis Mathematical Modeling and Computer Simulation, Science 219, 1281, 1983. 

Palusinski, OA Allgyer, TT Mosher, RA Bier, M Saville, DA, Mathematical Modeling and Computer Simulation of Isoelectric Focusing with Electrochemically Defined Ampholytes, Biophysical Chemistry 13, 193, 1981. 

The paper describes some applications to large scale protein fractionation using a recycling isoelectric focusing apparatus. Separation is achieved in free solution without the use of supporting media. Various alternatives for the formation of the pH gradient are discussed and results of a computer simulation are presented. 

Thormaim, W., Huang, T., Pawliszyn, J., and Mosher, R.A., High-resolution computer simulation of the dynamics of isoelectric focusing of proteins, Electrophoresis, 25, 324, 2004. 

Shimao, K., Computer simulation of electrophoretic transport. II. Modified general procedure and application to isoelectric focusing of proteins using immobilised pH gradient, Jap. J. Electrophor., 38, 221, 1994. 

Figures Computer-simulated distributions of 140 carrier ampholytes and of three dyes after 12, 100, 500,1000, 2000, and 5000 min of current flow. The numbers refer to the pi values of the dyes and the arrowheads point to their locations. Successive graphs are presented with a y-axis offset of 30 mmol 1 1 The arrows at the bottom graph mark the two transient concentration valleys that are characteristic for the stabilizing phase. The cathode is to the right. (Reproduced with permission from Mosher RA and Thormann W (2002) High-resolution computer simulation of the dynamics of isoelectric focusing using carrier ampholytes The postseparation stabilizing phase revisited. Electrophoresis 23. 1803-1814.)...

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