Complex formation with Coomassie blue

Figure 1. Formation of a cross-linked ETF MCAD complex. ETF (9.5pM), MCAD (38 pM) and octanoyl-CoA (l.SmM) were preincubated in 20 mM potassium buffer, pH 8.0 (lane C). ETF (18 pM) and MCAD (18 pM) were also incubated separately with ootanoyl-CoA in buffer (lanes A and B). After 3 mins DMS (9.5 mM) was added and the mixture incubated for 30 mins at 30 °C. After boiling, aliquots were subjected to electrophoresis on an 8% SDS polyacrylamide gel. Densitometric scans of the Coomassie Blue-stained gels are shown.

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