Complement experiments that can be performed

1 Experiments with enzymes from different sources 

The experiments described above can be repeated with enzymes from different sources, such as fungal amylase, amyloglucosidase, and a mixture of equivalent concentrations of three enzymes, bacterial amylase, fungal amylase, and amyloglucosidase. 

2 Sequential enzymatic treatment (corn syrup production) 

5 ml of the bacterial amylase solution to 50 ml of the 20 g 1 1 nonbuffered starch solution prepared in Step 1. Periodically place a few drops of the reaction mixture on a glass plate and add one drop of the iodine reagent. The color should finally turn red, indicating the total conversion of starch to dextrin. This liquefaction step should last for approximately 10 min. 

When the process of liquefaction is complete, adjust the pH of the starch solution to 4.7 with 1 N HC1. Filter the starch solution if it is turbid. Separate the solution into two equal parts. To the first starch solution, add 0.5 ml of amyloglucosidase to the second solution, add 0.5 ml of fungal amylase solution. 

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