Competition-in-solution assays

Competition-in-solution assays can quantify the effect of library components on the interaction between components of a protein complex. These assays include traditional biochemical assays that measure interactions through antibody- or tag-mediated pull-down or label displacement assays, along with biophysical methods that measure changes in the property of a component upon binding to its partner protein, such as fluorescence spectroscopic assays and SPR. It is essential for assay development that the requirements (affinity and binding site) for assembly of the interaction partners be at least partially defined such that a tractable model system for the target interaction can be developed, as was the case for MDM2/p53, IL-2/IL2-aR and Bcl/Bak [138-140],... 

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