Combined protein, albumin-globulin

Heparinoids and mucopolysaccharides react with, and modify, many of the plasma proteins. Heparin combines with fibrinogen, globulins and albumin. As judged by electrophoresis and various types of analysis and staining, the particular plasma protein components with which heparin combines are dependent upon the concentration of protein, concentration of heparin, pH value, and salts present. This explains the somewhat contradictory statements in literature about combinations of heparin with plasma proteins. The combination may result in change of solubility of the protein and reverse protein tests . Heparin can modify the murexide reaction for calcium in serum by affecting the calcium-protein-heparin complex. Many heparinoids... 

Figures 16a-d show the surface appearances of copper-2% zinc exposed to albumin, globulin, fibrinogen, and combination protein solutions, respectively, at 200 kHz. All surfaces represented desorbed protein states.

An indirect polarographic behavior of proteins may be seen in the possibility of tracing the alterations in the patterns of the diffusion currents and the half-wave potentials of different azo-dyes, alone or in combination with antibodies, globulins, and other nonreaginic proteins (e.g., albumins) for example, the hapten-antibody reaction could be followed by the derivations in the pattern of the polarographic reduction of the dyes, induced by the presence of antibody proteins in the reacting mixture (41). 

In order to obtain information about the polarographic properties of the individual protein components in blood sera, the polarography was combined with paper electrophoresis. After electrophoretic separation, the cut strips of paper with separated fractions of albumins and globulins are eluted in physiological sodium chloride solution and each sample is analyzed polarographically. These combined methods were applied for study of various pathological cases [147]. 

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