Combinatorial libraries unbiased

The DOFLA has advantage in the following aspects (Table 17.1). This approach benefits from the combinatorial chemistry techniques, once an efficient synthetic route can be developed for a diverse set of dyes. Thus broader chemical space could be explored and unknown/unexpected molecular interactions might be discovered. The design and preparation of the fluorescent dye library is unbiased to any specific target analyte, and the library would be evaluated with quite distinct analytes to maximize the chance of application in different fields. Taken all these together,... 

Only a few general points are addressed here. The reader is referred to a recent review by Gopinath (2007) for details on procednres for the selection of aptamers. Like any other combinatorial method, the SEFEX methodology first reqnires synthesis of the library. Compared to other libraries, it is easy to prepare an unbiased pool of DNA sequences, as the conpling efficiency of the A, T, G, or C phosphoramidite is very similar. One conld compensate for the slightly different incorporation of nucleotides a mixture of phosphoramidites in a ratio of... 

---