Comamonas Nil

The Comamonas Nil activities were determinated on AdN and four putative intermediates 5-cyanovaleramide, CVA, adipamate and adipamide (Table 3). 

Table 3. Comamonas Nil activity on adiponitrile, ammonium 5-cyanovalerate, 5-cyano valeramide, ammonium adipamate and adipamide...

The activity of these different recombinant strains are listed in Table 6 and compared to those of the wild Comamonas Nil. 

The activities of recombinant strains have confirmed the intensities of the bands on SDS-PAGE (data not shown). Compared to the wild strain Comamonas Nil, the activity was 160 times higher on AdN and 30 times on CVA. The different ratio between the rates on CVA and AdN was due to the presence of other enzymes which catalyse the transformation of CVA into AdO. The chemo-selectivity of recombinant strains was too weak to develop an industrial process. 

In recent years nitrile hydratases have been studied intensively. Enzymes from a wide range of microorganisms have been isolated and characterized and the corresponding genes have been cloned and overexpressed. Nitrile hydratases can be subdivided into two classes enzymes requiring Fe3+ (for example from Rhodococcus sp.(9 12, Pseudomonas chlororaphis B231131 and Comamonas testosteroni Nil[u ) and... 

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