Columns precautions related

Precautions regarding the use of the analytical columns, as well as the operation of each module, e.g., the pump, the detector, etc., may help keep away or postpone most of the abovementioned problems related to HPLC instmmentation. 

In order to make the best use of the technique, it helps to have some knowledge of the theory of gas chromatography, and this is outlined in Chapter 3. Many important aspects of chromatography are governed by a few simple equations, so a highly detailed account of the mathematics and physics of the subject is not necessary here. Novices on the subject or newcomers to capillary gas chromatography are confronted by a bewildering array of different columns, injection systems and detectors, and these are discussed systematically in relation to specific analytical problems. Various precautions are described to extend column life, and to ensure reproducibility in quantification, and details of these obviously cannot be repeated in each of the subsequent Chapters. 

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