Collecting bone marrow BM harvests for CD34 quantitation

4 Collecting bone marrow (BM) harvests for CD34 quantitation [Pg.379]

All BM harvests are processed to huffy coat to reduce the volume (Protocol 2) before FACS analysis (Protocol 1). [Pg.379]

The apparatus for large scale preparation of BM is essentially a centrifuge in which a processing bag (P) occupies the entire bowl when full of liquid. A central line from P passes through a hole in the hd and is attached by a T-junction to five colour-coded lines. These lines connect the jimction to transfer (T) and a [Pg.379]

Preparation of buffy coat from bone marrow harvests (BM) using a COBE 2991 ceii processing machine [Pg.380]

50 ml syringes and 25 gauge needles PBS (Baxter Health Care Corporation) Heparinized bone marrow harvest [Pg.380]

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