Collagenases metals

Enzyme Molecular weight pi Collagenase activity M- min ) Metal content (moljmol protein ) ... 

An aspect of these collagenases that bears directly on inhibition studies is that of their activation mechanism(s). In vitro studies have shown that pro-HFC and pro-HNC, as well as the other pro-MMP, can be activated by a variety of seemingly disparate means, including treatment with protein-ases, heavy metals, oxidants, disulphide compounds, alkylating agents, and conformational perturbants. The molecular basis for the latency of these zy-... 

Matrix Metalloproteases. Matrix metal-loproteases (MMPs) are a large and diverse family of zinc endoproteases. Several members of this family (such as the collagenases and the stromelysins) are thought to have important roles in proliferative diseases, including arthritis, retinopathy, and metastatic in-... 

Metalloprotease inhibitors - also known as metalloproteases or zinc proteases - are proteolytic enzymes of which the activity depends on metal ions, normally bound Zn -. Examples of metalloproteases are the pancreatic enzymes carboxypeptidase A and B, elastase, the well-characterized bacterial enzyme thermolysin and the collagenase family (found in both bacterial and mammalian cells, fibroblast collagenase, neutrophil elastase, gelatinase). 

Metallopeptidases are the most diverse of the catalytic types of proteases and are characterized by the requirement for a divalent metal ion for their activity (Barett, 1995). They include enzymes from a variety of origins sueh as collagenases from higher organisms, hemorrhagic toxins from snake venoms and thermolysins from bacteria (Weaver et al., 1977 Hibbs et al., 1985 Okada et al., 1986 Wilhelm et al., 1987). Based on the specifieity of... 

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