Coenzyme reorientation

Reorientations of the coenzyme associated with the enzymatic catalytic cycle were first proposed by Ivanov and Karpeisky64 for aspartate transaminase on the basis of general topochemical considerations and studies of induced optical activity. Torchinsky and Koreneva65,66 found that induced optical activity of PLP bound to the transaminase is... 

Amides can form fluorescent ternary complexes with reduced coenzyme 375,Ji.07) similar to those formed with LADH (Section II,H,2,b). The ternary complex with isobutyramide has been investigated using NMR methods (329), resulting in a determination of the position of the isobutyramide molecule with respect to the coenzyme. The results suggest a slight reorientation of the NADH molecule when the inhibitor binds. From measurements of the same complex with a cobalt containing YADH, a mean distance of about 7 A has been calculated from cobalt to the CHa protons of the isobutyramide molecule (44 )- These results are consistent with the coordination of the inhibitor molecule to zinc. 

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