Cocktails and Mixtures

For the past decade, compound collections have generally been screened as discrete compounds with a focus on high purity in order to generate reliable hits. However, it should be noted that there may also be advantages of using cocktails or mixtures for screening. 

cocktails of pure, high-quality compounds will reduce the number of screening points and will, therefore, reduce cost and increase speed. A full HTS of 64 000 compounds toward human cysteine protease cathepsin B has been compared with a screen with 10 compounds per well, which had been orthogonally selected [175]. The results of both strategies were comparable. 

Second, in positional scanning synthetic combinatorial libraries (PS-SCL), a number of different residues on the same attachment points can be synthesized 

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