Clostridium ramosum

However, several disadvantages can be reported. This PCR method requires two different PCR systems mollil + molli2a to detect all the Mycoplasma and Spiroplasma spp. and mollil + molli2b to detect Acholeplasma spp. The set of primers mollil + molli2a amplifies two phylogeneticaUy closely related species -Clostridium ramosum and C. inocuum - but these species are rarely found as cell culture contaminants. Moreover, this method does not allow bacterial identification of the detected species or antibiotic susceptibility testing. 

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