Clostridium difficile ToxB

Fig. 3. Actinomorphic effect induced by Clostridium difficile ToxB in UDP-GIc-deficient mutant cells after microinjection of UDP-GIc. Chinese hamster lung mutant cells (Florin, 1991) were treated with ToxB (12ng/ml) for 1 h, after which some cells were microinjected with UDP-GIc (100mM). The microinjected cells developed the characteristic actinomorphic effect (Chang ef al., 1978) within 30 min, whereas non-injected cells (arrows) were unaffected (Chaves-Olarte et al., 1996)...

Fig. 1. Purification of Clostridium difficile foxin A and toxin B by FPLC chromatography. 20 ml of the resuspended and dialyzed ammonium sulfate precipitate of the culture supernatant was loaded onto a MonoQ HR 10/10 column (Pharmacia, Freiburg, Germany). Elution was performed wifh a linear NaCI gradient (0.05 to 1.0M) at a flow rate of 1 ml/min. Extinction was measured at 280 nm in arbitrary units with an amplification factor of 0.5. The toxins eluted at either 25 % buffer B (1 M NaCI / 0.05 M Tris-HCI pH 7.5) tor toxin A (ToxA) or at 60 % buffer B tor toxin B (ToxB)...

---