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Clostridium difficile ToxA

Just I, Wilm M, Selzer J, Rex G, von Eichel-Streiber C, Mann M, Aktories K The enterotoxin from Clostridium difficile (ToxA) mono-glucosylates the Rho proteins. J Biol Chem 1995 270 13932-13936. [Pg.33]

Fig. 1. Purification of Clostridium difficile foxin A and toxin B by FPLC chromatography. 20 ml of the resuspended and dialyzed ammonium sulfate precipitate of the culture supernatant was loaded onto a MonoQ HR 10/10 column (Pharmacia, Freiburg, Germany). Elution was performed wifh a linear NaCI gradient (0.05 to 1.0M) at a flow rate of 1 ml/min. Extinction was measured at 280 nm in arbitrary units with an amplification factor of 0.5. The toxins eluted at either 25 % buffer B (1 M NaCI / 0.05 M Tris-HCI pH 7.5) tor toxin A (ToxA) or at 60 % buffer B tor toxin B (ToxB)... Fig. 1. Purification of Clostridium difficile foxin A and toxin B by FPLC chromatography. 20 ml of the resuspended and dialyzed ammonium sulfate precipitate of the culture supernatant was loaded onto a MonoQ HR 10/10 column (Pharmacia, Freiburg, Germany). Elution was performed wifh a linear NaCI gradient (0.05 to 1.0M) at a flow rate of 1 ml/min. Extinction was measured at 280 nm in arbitrary units with an amplification factor of 0.5. The toxins eluted at either 25 % buffer B (1 M NaCI / 0.05 M Tris-HCI pH 7.5) tor toxin A (ToxA) or at 60 % buffer B tor toxin B (ToxB)...
See other pages where Clostridium difficile ToxA is mentioned: [Pg.168]    [Pg.168]    [Pg.141]   


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