Clinical endpoints overview

Table 2.1 Overview of evidence for risk estimate of several clinical endpoints from randomized clinical trials with B vitamins. -...

However, real-time detection requires access to a special real-time PCR cycler, which is able to detect the increase/decrease of added fluorescence labels during DNA amplification. Although these machines are more and more common for quantitative DNA analysis, their availability in clinical laboratories is still limited. Therefore, the following subsections also include a detailed overview of the classical approaches to quantitative (I)PCR amplificate, analysis which exchanges less demanding PCR equipment for additional hands-on time. The sensitivity of real-time or end-point IPCR detection is quite similar. A comparison of the influence of different endpoint detection methods to the overall sensitivity of IPCR is given in Fig. 5. 

Wassmer G, Reitmeir P, Kieser M, Lehmacher W (1999) Procedures for testing multiple endpoints in clinical trials an overview. Journal of Statistical Planning and Inference 82 69-81. 

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