Classic bioaffinity chromatography

The rate of equilibrium attainment mainly during the batchwise arrangement is strongly affected by the concentration of a substance to be isolated in the sorption solution. Fig. 4.7.6 shows the rate of adsorption of trypsin on to soybean trypsin inhibitor-agarose particles in suspensions in solutions containing equal amounts of trypsin but of a different concentration [129]. The rate of sorption is determined on the basis of the residual activity of trypsin measured at various time intervals. 

Although simple batch processing can be used in appijnng a biospecific ad- 

Effect of cycling a 5-ml column with excess antigen and eluting with 15 ml of 2.5 M NH4SCN 

Cycle Amount (mg) Eluted/bound (%) Amount retained (mg) 

---