Class 2 mannosidases

Enzymes that fall within the category of Class 2 mannosidases are distinguished from Class 1 mannosidases by similarities in size of their catalytic region (110-116 kDa). Although the sizes of the proposed catalytic domains of the Class 2... 

K. Karaveg, A. Siriwardena, W. Tempel, Z.-J. Liu, J. Glushka, B.-C. Wang, and K. W. Moremen, Mechanism of class 1 (glycosylhydrolase family 47) a-mannosidases involved in iV-glycan processing and endoplasmatic reticulum quality control,./. Biol. Chem., 280 (2005) 16197-16207. 

F. Vallee, K. Karaveg, A. Herscovics, K. W. Moremen, and P. L. Howell, Structural basis for catalysis and inhibition of /V-glycan processing class I a-l,2-mannosidases, J. Biol. Chem., 275 (2000) 41287-41298. 

N. Shah, D. G. Kuntz, and D. R. Rose, Comparison of kifunensine and 1-deoxymannojirimycin binding to class I and II a-mannosidases demonstrates different distortions in inverting and retaining catalytic mechanisms, Biochemistry, 42 (2003) 13812-13816. 

Lobsanov, Y. D., Vallee, F., Imberty, A., Yoshida, T., Yip, P., Herscovics, A., and Howell, P. L. (2001). Structure of Penicillium citrinum al,2-mannosidase reveals the basic differences in specificity of the endoplasmic reticulum and Golgi class Ienzymes. J. Biol. Chem., 277, 5620-5630. 

Lipari, F., and Herscovics, A. (1999). Calcium binding to the class I alpha-1,2-mannosidase from Saccharomyces cerevisiae occurs outside the EF hand motif. 

Johnston, B D, Pinto, B M, Synthesis of 1,2- and 1,3-A-linked disaccharides of 5-thio-a-D-mannopyranose as potential inhibitors of the processing mannosidase class I and mannosidase II enzymes, J. Org. Chem., 63, 5797-5800, 1998. 

Based on its primary sequence, mannosidase II is a member of the class II mannosidase family (CAZy glycohydrolase family 38, which includes also the lysosomal mannosidase involved in glycoprotein catabolism). Like the lysosomal enzyme, mannosidase II is inhibited by swain-sonine [62], thus this reagent can be used to block the formation of complex A-glycans. The... 

Mannosidase Class Class 1 Class 2 Class 1 Class 1 Class 2 Class 2 Class 2 Class 2... 

Class 2 Mannosidases enzymes of the cytosol, ER, Golgi, and lysosomes... 

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