Cinchona robusta suspension culture

Schripsema, J Ramos,V.A Verpoorte, R. Robustaquinones, novel anthraquinones from an elicited Cinchona robusta suspension culture. Phytochemistry. 1999, 51(1), 55-60. 

Ramosvaldivia, A.C., Vanderheijden, R. and Verpoorte, R. (1997a) Elicitor-mediated induction of anthraquinone biosynthesis and regulation of isopentenyl diphosphate isomerase and famesyl diphosphate synthase activities in cell suspension cultures of Cinchona robusta How. Planta, 203, 155-61. 

Interesting correlation between distribution of the strictosidine synthase activity and alkaloid content in the 6-month C. ledgeriana plants was found by Aerts et al. The enzyme was active in the top of stems, in young leaflets, and in the roots, and these parts also contained the highest concentration of the alkaloids. Quinoline alkaloids were accumulated in the roots, whereas cinchophyllines in aerial part of plants [218]. Similarly high activity of strictosidine synthase in the germinating Cinchona seeds and cell cultures has been reported [219]. Four isoforms of strictosidine synthase have been isolated and purified from the suspension culture of C. robusta [220]. 

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