Chymotrypsin mimetics

Furthermore, phenyl ester are also suitable substrate mimetics for chymotrypsin-catalyzed peptide synthesis, as was established by Bordusa s group and will demonstrated by sophisticated fragment condensations in Sect. 12.5.3.7. 

Figure 12.5-28. Chymotrypsin-catalyzed (8+16) segment synthesis ofthe Ht 31 (493-515) peptide via substrate mimetic strategy12331.

Chymotrypsin and V8 peptidase, members of the trypsin family of serine proteases, as well as an engineered trypsin variant (D189K/K60E), were employed for the N-terminal introduction of 2-aminobenzoyl- and 3-(4-hydroxy-phenyl)-propionyl groups, and biotin in the form of substrate mimetics into peptides [115]. 

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