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Chromosomes enzymatic methods

The resulting transformants are antibiotic sensitive, which is a convenient screening criterion for correct chromosomal integration. The method has been used to replace native, regulated promoters with strong constitutive promoters for genes and operons involved in the biosynthesis of riboflavin [135], biotin [176], pantothenate [134,177], folate, and thiamin [178]. This method can also be used to introduce point mutations, as long as sufficient enzymatic activity remains to allow for selection of prototrophic colonies [179,180]. [Pg.239]

See other pages where Chromosomes enzymatic methods is mentioned: [Pg.405]    [Pg.636]    [Pg.162]    [Pg.421]    [Pg.493]    [Pg.313]    [Pg.397]    [Pg.312]    [Pg.222]    [Pg.146]    [Pg.592]    [Pg.913]   
See also in sourсe #XX -- [ Pg.153 , Pg.154 , Pg.155 , Pg.156 , Pg.157 , Pg.158 ]



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Enzymatic methods

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