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Chromatography electrostatic interactions

Stahlberg,., Jonsson, B., and Horvath, Cs., Theory for electrostatic interaction chromatography of proteins, Anal. Chem., 63, 1867, 1991. [Pg.270]

Stahlberg J, Appelgren U, Jonsson B (1995) Influence of charge regulation in electrostatic interaction chromatography of proteins. J Colloid Interf Sci 176 397 107... [Pg.123]

Figure 6.11 Principle of ion-exchange chromatography, in this case anion exchange chromatography. The chromatographic beads exhibit an overall positive charge. Proteins displaying a nett negative charge at the pH selected for the chromatography will bind to the beads due to electrostatic interactions... Figure 6.11 Principle of ion-exchange chromatography, in this case anion exchange chromatography. The chromatographic beads exhibit an overall positive charge. Proteins displaying a nett negative charge at the pH selected for the chromatography will bind to the beads due to electrostatic interactions...
Efficiency, of commercial columns, 40 Eicosyltrichlorosilane, 133 Electrophoresis, comparison to RPC, 224 Electrostatic interactions, 120, 208, 213 calculation of 208-211 dipoles, 208, 209 at high ionic strength, 209-210 in ion-pair chromatography, 238 at low ionic strength, 209 monopoles, 209-211... [Pg.166]

Many pharmaceutical preparations contain multiple components with a wide array of physico-chemical properties. Although CZE is a very effective means of separation for ionic species, an additional selectivity factor is required to discriminate neutral analytes in CE. Terabe first introduced the concept of micellar electrokinetic capillary chromatography (MEKC) in which ionic surfactants were included in the running buffer at a concentration above the critical micelle concentration (CMC) [17], Micelles, which have hydrophobic interiors and anionic exteriors, serve as a pseudostation-ary phase, which is pumped electrophoretically. Separations are based on the differential association of analytes with the micelle. Interactions between the analyte and micelles may be due to any one or a combination of the following electrostatic interactions, hydrogen bonding, and/or hydro-phobic interactions. The applicability of MEKC is limited in some cases to small molecules and peptides due to the physical size of macromolecules... [Pg.111]

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See also in sourсe #XX -- [ Pg.11 ]



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