Chromatography Acetyl Ferrocene, Cholesteryl Acetate, and Fluorenone

Column Chromatography Acetyl Ferrocene, Cholesteryl Acetate, and Fluorenone 

Column chromatography is one of the most useful methods for the separation and purification of both solids and liquids when carrying out small-scale experiments. It becomes expensive and time-consuming, however, when more than about 10 g of material must be purified. 

The application in the present experiment is typical a reaction is carried out, it does not go to completion, and so column chromatography is used to sepcu ate the product from starting material, reagents, and byproducts. 

Three mutual interactions must be considered in column chromatography the polarity of the sample, the polarity of the eluting solvent, and the activity of the adsorbent. 

The activity of the alumina on TLC plates is usually about III. Silica gel for column chromatography, 70-230 mesh, has a surface area of about 500 mVg and comes in only one activity. 

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