Chromatogram tissues

Chromatogram Showing Histamines from Rat Brain Tissue... 

The dried chromatogram is first dipped in reagent solution 1 for 1 s, dried briefly in a stream of cold air and then dipped in reagent solution 2 for 1 s. The TLC/HPTLC plate is then held upright on tissue paper to allow excess reagent to drain away when the layer appears matt it is covered with a glass plate and kept at room temperature for 5 min. Afterwards it is dried in a stream of hot air and exposed to ammonia vapor. 

Figure 4A. Chromatogram illustrating the studies conducted on the enzymatic conversion of the PSP toxins to decarbamoyl metabolites (appended with an "M" in these figures). Conversion of Cl to dcGTXII (GTX IIM) in a homogenate of littleneck clam tissue after 4 and 48 hr.

Tissue samples obtained from the different colored regions of the larvae were separately analyzed by HPLC. The white, black, and yellow bands of Monarchs all contained a single, major carotenoid component, lutein (all / -3,3 - d i h yd roxy-13, e - ca ro t e n e), Figure 25.3a. The amount of lutein present in the black and white bands was markedly lower ( 15x) than that in the yellow bands, see below. A small quantity of 13-m-lulein and zeaxanthin were observed to elute immediately following lutein in the chromatogram and the lutein peak was preceded by a unique metabolite that is formed by the cleavage of lutein, see Section 25.4. 

Figure 1. HPLC chromatograms of an authentic sample (0.255 lAg) of salvinorin A (A), and of representative Salvia divinorum tissue extracts obtained from "Palatable" clone (Bret Blosser) (B), from Cerro Rab6n clone (L. J. Valdes) (C), and from a seed grown plant DS03 (D. J. Siebert) (D). In each case the retention time of the peak representing salvinorin A is indicated. (For chromatographic protocol see Experimental section).

Fig. 29.4 Representative chromatograms of a tylosin standard solution (A), a blank muscle tissue (B), and a blank muscle tissue fortified with tylosin at a concentration of 50 ppb (C), and 400 ppb (D). (From Ref. 152.).

Fig. 29.6.1 Chromatograms obtained with UV and fluorescence detection from analysis of catfish tissue containing incurred quinolones. (Reprinted from Ref. 196. Copyright, (1995), by AOAC INTERNATIONAL.)...

Fig. 29.16 Chromatograms of (a) tissue extract spiked with seven sedatives and carazolol at 2 ppb and (b) mixed standard equivalent to 2 ppb. (Reprinted from Ref. 523, with permission from Elsevier Science.)...

Example chromatograms are shown in Figures 11.4.3 and II. 4.4. Generally speaking, epi-catechin extension subunits are the most prevalent subunits found in plant tissues of interest as foodstuff. Some additional references are given at the end of this unit that provide information on the subunit composition of various plant species. 

Figure 6. Total ion current chromatogram of endogenous and spiked pesticides extracted from an EPA standard fish tissue.

Figure 2. Radioactivity chromatogram of sulfur compounds derivatized with monobromobimane. The reversed-phase HPLC separation is based on the hydrophobic properties of the bimane-sulfur adducts but peak area is based on "S-radioactivity of the compounds. At time 0 sulfite and thiosulfate impurities are present before addition of the hepatopancrease tissue homogenate. This was a 60 min experiment to determine the sulfide detoxifying functions of the hepatopancrease of the hydrothermal vent crab Bythograea thermydron. During this time the proportion of radioactivity in sulfide rapidly decreases and thiosulfate and sulfate accumulate as end products. Two intermediates, pi and p2 accumulate then decrease during the experiment. The two intermediates are believed to be polysulfides based on similar elution times of polysulfide standards. (Figure is the unpublished chromatograms from the data in Vetter et al. (24)-) continued on next page.

FIGURE 5 (A) UHPLC ESI+chromatogram of the polar extract of postmortem brain tissue. (B) The scores plot displaying the separation between the two sam-... 

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