Sunflower, chromatogram

Fig. 1 Chromatogram of fatty oils (9 pg each per 10 mm band) after iodine vapor treatment (A) and after additional immersion in a starch solution (B) Itack 1 avocado oil, Hack 2 sunflower oil, Tlack 3 linseed oil, Tlack 4 almond oil.

Figure 6. Chromatogram obtained from liquid CO2 high pressure Soxhlet extraction of Eriophyllum staechadifolium (seaside woolly sunflower, also known as lizardtail). Conditions as shown in Figure 2.

Fig. 6. Partial gas chromatograms of saturated cyclic fatty acid methyl esters derived from (A) high-oleate sunflower oil and (B) normal sunflower oil. A Cp-Wax 52CB (25 m x 0.25 mm i.d., 0.20 pm film thickness) capillary column was used. There was an initial temperature of 170°C for 5 min followed by a program at 2°C/min to 210°C. Peaks q-x were monocyclic acids, and later eluting peaks had bicyclic structures. Source Ref. 7.

Figure 2.3 Supercritical fluid chromatogram with use of miniaturized evaporative light-scattering detector, of a mixture of cohune oil and sunflower seed oil (50 50). Column as in Fig. 2.2. Conditions injection at 115°C and 180 atm after 1 min, programmed at -1°C min to 75 C and at 2 atm min to 210 atm, then 4 atm min to 320 atm. Mobile phase carbon dioxide/ acetonitrile/isopropanol (92.8 6.5 0.7 mol%). Peaks and abbreviations as in Fig. 2.2.

Product Characterization. The products of sunflower leaf hydroperoxide lyase are cw-3-hexenal and 12-oxo-c/.y-9-dodecenoic acid. Analysis of the methoxylamine derivative of the volatile C -aldehyde product by GC-MS showed that it had a mass spectrum and GC retention time identical to that of the methyloxime derivative of commercial c/>3-hexenal, with a small molecular ion at m/z 127 and a large M-29 peak at m/z 98. In contrast, the methyloxime derivative of commercial rra/ij-2-hexenal had a large molecular ion at m/z 127 and a smaller M-29 peak (m/z 98), and it eluted later on the chromatogram. The oxoacid product, 12-oxo-ci>9-dodecenoic acid, was characterized as its methyloxime, methyl ester derivative. The compound had a molecular ion at m/z 255, with a larger M-31 fragment at m/z 224. Its GC retention time was earlier than that of a methoxylamine, methyl ester derivative of 12-oxo-rra/2j-10-dodecenoic acid standard, which had an M-31 fragment (m/z 224) smaller than the molecular ion at m/z 255. 

Figure 3 Typical HPLC chromatogram of the initial triacylglycerol (TAG) composition of sunflower seed oil. A magnification of the area in the vicinity of 39 min is also shown in the figure.

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