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Chromatin immunoprecipitation ChIP assay

Occupancy of TBP at promoters in vivo was examined by chromatin immunoprecipitation (ChIP) assay (Kuras and Struhl, 1999 Li et al., 1999). The results indicate that the level of TBP occupancy at promoters correlates well with transcription level and is stimulated by activators. Interestingly, TBP occupancy is dependent on Srb4, a subunit of the Pol II holoenzyme. This indicates that TBP recruitment is an important mechanism of transcriptional activation in vivo and is interdependent with holoenzyme recruitment. [Pg.79]

Current data from in vitro DNA binding assays and transfection experiments point to Nrf2 as the most important protein involved in stimulating ARE-driven transcription. Using the chromatin immunoprecipitation assay (ChIP) studies from... [Pg.242]

See other pages where Chromatin immunoprecipitation ChIP assay is mentioned: [Pg.169]    [Pg.212]    [Pg.1735]    [Pg.71]    [Pg.133]    [Pg.213]    [Pg.128]    [Pg.168]    [Pg.169]    [Pg.212]    [Pg.1735]    [Pg.71]    [Pg.133]    [Pg.213]    [Pg.128]    [Pg.168]    [Pg.282]    [Pg.204]    [Pg.309]    [Pg.30]    [Pg.462]    [Pg.463]    [Pg.377]    [Pg.360]    [Pg.213]    [Pg.248]    [Pg.473]    [Pg.89]    [Pg.218]    [Pg.205]   
See also in sourсe #XX -- [ Pg.168 , Pg.172 , Pg.173 , Pg.228 ]



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