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Cholesterol oxygenation

Tab. 6.8 Average number of hydrogen bonds per DPPC or cholesterol oxygen, formed with water molecules reported from several simulations. Data for DPPC and DPPC-cholesterol are taken from ref. 73 and data for DMPC-cholesterol sulfate are from ref. 75. Data for DMPC-cholesterol are taken from ref. 71 and data for pure DM PC are taken from ref. 74 and 106. Tab. 6.8 Average number of hydrogen bonds per DPPC or cholesterol oxygen, formed with water molecules reported from several simulations. Data for DPPC and DPPC-cholesterol are taken from ref. 73 and data for DMPC-cholesterol sulfate are from ref. 75. Data for DMPC-cholesterol are taken from ref. 71 and data for pure DM PC are taken from ref. 74 and 106.
Figure C1.5.17.(A) Enzymatic cycle of cholesterol oxidase, which catalyses tire oxidation of cholesterol by molecular oxygen. The enzyme s naturally fluorescent FAD active site is first reduced by a cholesterol substrate,... Figure C1.5.17.(A) Enzymatic cycle of cholesterol oxidase, which catalyses tire oxidation of cholesterol by molecular oxygen. The enzyme s naturally fluorescent FAD active site is first reduced by a cholesterol substrate,...
In the enzymatic assays of cholesterol, glucose, and urea, oxygen is used and H2O2 is formed. The reaction for uric acid [69-93-2] is... [Pg.39]

Cholesterol The end point for the cholesterol reaction can be determined by following dye formation. Additionally, the amount of oxygen consumed can be measured amperometricaHy by an oxygen-sensing electrode (see Electro analytical techniques). The H2O2 produced by cholesterol oxidase requires phenol to produce dye. [Pg.39]

The preparation of 7,7-d2-cholesterol in 1950 was the first example of deuterium incorporation into steroids via desulfurization of mercaptals with deuterated Raney nickel. A substantially modified version of this reaction subsequently became the first widely used method for site-specific insertion of two deuteriums in place of a carbonyl oxygen. This conversion consists of the preparation of a mercapto derivative (84 85), which usually... [Pg.171]

Subczynski, W. K., J. S. Hyde, and A. Kusumi. 1989. Oxygen permeability of phosphatidylcholine-cholesterol membranes. Proc. Natl. Acad. Sci. USA 86 4474-4478. [Pg.211]

Girotti, AW and Korytowski, W, 2000. Cholesterol as a singlet oxygen detector in biological systems. Methods Enzymol 319, 85-100. [Pg.343]

Trettnak W., Wolfbeis O.S., Fiber optic cholesterol biosensor with an oxygen optrode as the transducer Anal. Biochem. 1990 184 124. [Pg.44]


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Oxygenated derivatives of cholesterol

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