Cholate dialysis method

FqF, proteoliposomes can be reconstituted by the cholate dialysis method [123] or another method [124]. The resultant reconstituted FqF may have two different orientations in liposomes, but since the membranes are not permeable to ATP,... 

Many different methods [340] are available for the reconstitution of bacteriorhodopsin into liposomes (a) sonication of purple membrane with dried, dispersed lipids [341] (b) co-precipitation of lipids and bacteriorhodopsin from organic solvents, such as dimethylsulfoxide [340] (c) dispersion of purple membrane sheets or monomeric bacteriorhodopsin and lipids in detergents, such as Triton X-100 [340], cholate [17,325], deoxycholate [170], or octyl-glucoside [327,342], followed by dialysis or removal of the detergent with Bio-Beads. 

Other conversion methods depend on the disruption of micelles containing water-insoluble compounds. For example, lipids e.g. lecithins can be reasonably well cosolubilized in micelles of sodium cholate or SDS. Dialysis slowly removes monomeric detergent molecules which dissociate away from the micelle resulting in a more concentrated lipid. Time and temperature controlled dialysis of the micelles finally yields monolamellar vesicles of uniform radii. ... 

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